Unmethylated Wnt antagonists compared with patients with With a better prognosis in non-small cell lung cancer patients with It is well established thatĭysregulation of these two pathways frequently leads to Signaling pathways are considered to serve key roles in embryonicĭevelopment and cell proliferation. Various studies have reported that EGFR and Wnt signaling cascadesĪre associated with and conserved in neoplasia. Globe searching for novel therapy agents ( 11– 13). Interest as a potential target from a number of studies across the Receptor-tyrosine kinase (EGFR-TK) was aberrantly overexpressed inīreast cancer ( 9, 10). Previous studies reported that epidermal growth factor Therefore, it is vital to identifyĭrugs that are able to target novel sites and act in a specific Its efficacy has been seriously compromised due to the development Progression of breast cancer is the recommended option, however,
Therapeutic modalities, current options to manage breast cancer Gradually increasing worldwide since 2008 ( 4). Previous data suggested the number of breast cancer cases has been Mortality rate is >450,000 individuals per year ( 3).
Based on the findings of the current study, the chemical 1,3,5‑triazine series are potential novel inhibitors of EGFR‑TK and β‑catenin signaling, and may be potent anti‑breast cancer agents.Ĭancer-associated mortality in women ( 1, 2). Western blot analysis was performed to examine the levels of β‑catenin in the control and treated cells. Compound 1d was further evaluated for its effect on the cellular viability of three breast cancer cell lines, including highly metastatic MDAMB231, human epidermal growth factor receptor 2‑positive BT474 and estrogen receptorpositive MCF7 cells, using an MTT assay and apoptosis analysis. Compound 1d was demonstrated to be the most potent analogue with an inhibitory constant of 0.44 nM, against EGFR‑TK in in‑vitro enzyme inhibition assay. The molecules were demonstrated to interact with key catalytic residues of EGFR, including Asn818, Lys721, Leu694, Val702 and Met742 as demonstrated in molecular docking experiments. The results suggested that 1,3,5‑triazine derivatives exhibited optimal drug likeness via molinspiration and proved to be effective drug candidates as potential anticancer agents. The present study investigated the effect of certain 1,3,5‑triazine derivatives on epidermal growth factor receptor‑tyrosine kinase (EGFR‑TK).